The series starts out with Tsukune Aono, an average teenager, is unable to get into any local high schools due to his poor grades. However, his parents are happy to have him enroll in a private school called Yokai Academy (which requires no entrance exam). When he arrives, he discovers that Yokai is in a remote location, and is actually a boarding school for monsters who are being taught how to coexist with humans, including disguising themselves as them, and that any real humans who discover its existence are to be killed. Tsukune fears for his life, but makes friends with a beautiful vampire girl Moka Akashiya, who gains an obsession for his blood. He later meets other monster girls who though usually starting out as antagonist, eventually take a romantic liking to him.
Rosario + Vampire is followed by Rosario + Vampire Capu2.